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jwb
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V 27
2020-11-15T18:50:17.968979Z
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V 837
biology/pooler: Optimise DNA sequencing primer-set combinations

Optimise combinations of primers and minimise the formation of dimers in
multiplexed PCR.

Primer Pooler can:

* Check through each proposed pool for combinations that are likely to form
  dimers

* Automatically move prospective amplicons between proposed pools to reduce
  dimer formation

* Automatically search the genome sequence to find which amplicons overlap, and
  place their corresponding primers in separate pools

* Optionally keep pool sizes within a specified range

* Handle thousands of primers without being slow (useful for high-throughput
  sequencing applications)

* Do all of the above with degenerate primers too.

WWW: http://ssb22.user.srcf.net/pooler/

PR:             ports/251065
Submitted by:   Silas S. Brown <silas-freebsd@flatline.org.uk>

END